Nucleofection keluaran terbaru

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09-2005 optimized for use with the UC06 cell line. Culture conditions before nucleofection The cells should be passaged 2 - 3 days before nucleofection.

Nucleofection is a method of delivering nucleic acid to the cells by creating transient small pores in the cell membrane by applying an electric pulse. The Neon system uses a simple 3-step transfection procedure. In addition nucleofection is a fast method because the results can be analyzed within 7 h.

09-2005 optimized for use with the UC06 cell line.

The Neon system uses a simple 3-step transfection procedure. The rate of nucleofection is quite acceptable 40 GFP positive and two days post-nucleofection upon observing T cell colonies which seem healthy I start selection with puromycin with 2. Customization of these two parameters has been proven to result in highly efficient gene transfer even in hard-to-transfect cells. Higher cell densities may cause lower Nucleofection Efficiencies Trypsinization 16 Remove media from the cultured cells and wash cells once with PBS.